UVP ChemiDoc-It TS2 Imagers - Manual (Page 15)

GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 15
Active Camera: The name of the installed camera will be displayed in this area. For example, in this
case the current camera is the Chemi 415 camera for the ChemiDoc-It TS2 Imager.
Inactivity Light Shutoff: The Inactivity Light Shutoff will automatically turn off the transilluminator within
the darkroom after a preselected period of time. From the drop down menu, select from 1, 5, 10,
15, 20, 25 or 30 minutes of inactivity time prior to shutoff. To re-enable lighting, access the
Lighting and Filters menu from the main screen to turn the lighting off and back on again.
Binning: Binning is the process of combining multiple image pixels into one larger pixel. For example,
2x2 binning combines a two-by-two area of pixels (four pixels all together) into one large pixel, 4x4
binning combines a four-by-four area of pixels (sixteen pixels all together) into one large pixel, and
so forth. This process shortens the image preview and capture process when increased sensitivity
is needed, and also aids the camera’s CCD sensor in capturing and displaying more image signal
in low-light situations (such as in chemiluminescence applications). The disadvantage of binning is
an overall reduction of resolution, as the effective image pixel area is exponentially reduced as
binning is increased.
Select a higher Preview binning to increase the preview frame rate and make sample previewing
faster. Preview binning will not affect the captured image. (Note: Preview binning is set to 4x4 by
default.)
Select a higher Capture binning to decrease image capture time or to acquire more image signal in
the same amount of time, especially in low light situations. Note that increased capture binning will
decrease the overall pixel resolution of the captured image. (Note: Capture binning is set to 1x1,
thus no binning, by default.)
Turn ON the Compensate Exposure for Binning setting when Preview and Capture binning
settings are not the same. This will automatically adjust the preview exposure time to provide a
preview image to match what will be displayed upon final image capture.
Post-Processing Tab
Rotate: To rotate the image, touch the down arrow and choose to rotate the image 90 degrees
clockwise, 90 degrees counterclockwise, or 180 degrees (upside down).
Auto Rotate: Set Auto Rotate to ON to automatically rotate the image to the desired degree upon
image capture.
Auto Invert Image: Set Auto Invert Image to ON to automatically invert the image upon image capture.
Noise Subtraction: Set Noise Subtraction to ON to reduce the amount of background and ambient
(“white”) noise within the image. In most circumstances, this setting should be left ON.
Autocapture Image Contrast: Select from Best (longer exposure), Better, Good and Minimum (fast
exposure) for images acquired using Auto Capture on the main TS2 screen. Best (longer
exposure) exposes the image to the maximum value of the histogram (65,000 gray levels). Better
exposes to fill the histogram 50% so the brightest portion image is at 32,000 gray levels. Good
exposes to fill the histogram to 25% or 16,000 gray levels. Finally, Minimum (fast exposure)
exposes to fill the histogram to 10% over background. Minimum and Good settings are particularly
useful for chemiluminescent imaging applications and allow for quicker image capture overall.
NOTE: Always set Auto Adjust to ON when using Better, Good and Minimum settings. See the
“Identifying the Touch Screen Buttons and Functions” section of this manual for more information
about Auto Adjust.