Selecting emission filters, Using the transilluminator, Using the overhead (epi) lighting – UVP ChemiDoc-It TS2 Imagers User Manual

Page 12: Using the uv gel viewer window, Image focusing

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GelDoc-It

TS2

and ChemiDoc-It

TS2

Imagers

12

Selecting Emission Filters

See the “Installing Emission Filters” section of this manual for instructions on installing individual emission
filters.

Once all filters have been installed, select the Lighting and Filters menu in the TS2 Software and click the
Reset Wheel button under the “Filters” tab to realign and calibrate the filter wheel within the system.

Using the Transilluminator

To use the UV transilluminator or LED White Light Plate, ensure that the components are plugged into the
system (see the “Connecting the Power Cables” section of this manual for more information).

Using the Lighting and Filters menu in the TS2 Software, select either UV or White Light Plate depending
upon the application. When using the UV transilluminator, ensure that the green power switch on the front of
the transilluminator unit is in the ON position.

NOTE: For UV protection and to extend the life of the UV transilluminator, the system incorporates a
customizable transilluminator shutoff timer built into the software. For additional information, refer to the TS2
Software User Manual included with the system or available online at http://uvp.com/manuals.

Using the Overhead (Epi) Lighting

To operate the built-in overhead white light, use the Lighting and Filters menu in the TS2 Software and
select White under Epi Illumination.

To operate the optional overhead (epi) UV illumination, ensure that the optional UV handheld lamps are
plugged into the back of the darkroom interior and are mounted to the brackets at the top of the darkroom.
Also ensure that the power switches, located on the handheld lamps, are in the ON position. To provide
power to the handheld lamps, use the Lighting and Filters menu in the TS2 Software and select
Accessory under Epi Illumination.

Using the UV Gel Viewer Window

The UV Gel Viewer Window, built into the darkroom door, allows users to view the interior of the darkroom
without opening the darkroom door.

To open the Window, press firmly on the top of the Window cover to release the pressure-sensitive clasp
and open the viewer. The Window glass is UV blocking while providing a clear view to the transilluminator
surface for sample viewing without opening the darkroom door.

NOTE: Close the UV Gel Viewer Window prior to capturing a light-sensitive image such as a
chemiluminescent blot.

Image Focusing

Prior to capturing an image, prepare the image focus:

1. Turn on the transilluminator and place the Fluorescent Focus Target (see the “Fluorescent Focus

Target” section of this manual for more information) on the transilluminator surface. NOTE: The
darkroom has a UV safety switch that turns off the transilluminator and optional UV handheld lamps
when the door is open.

2. Using the TS2 Software, click to turn ON the “Live Preview”. Adjust the exposure, aperture, zoom

(if applicable) and focus controls on the bottom portion of the TS2 Software screen until an ideal
image is visible.

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