Metrohm 940 Professional IC Vario ONE/SeS/PP/Prep 2 User Manual
Page 114
6.1
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104
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940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)
Problem
Cause
Remedy
Note: 6.2821.180 pump tubing connection
with filter must be used.
Guard column – Blocked.
Replace the guard column (see Chapter 3.22,
page 56).
Separation column –
Blocked.
￭
Regenerate the separation column (see
Chapter 5.17.4, page 101).
￭
Replace the separation column (see "Con-
necting the separation column", page 60).
Note: Samples should always be microfiltered .
Injection valve – Valve
blocked.
Have the valve cleaned (by a Metrohm service
technician).
The retention times
in the chromato-
gram have changed
unexpectedly.
Separation column –
Diminished separating effi-
ciency.
￭
Regenerate the separation column (see
Chapter 5.17.4, page 101).
￭
Replace the separation column (see "Con-
necting the separation column", page 60).
The eluent contains gas
bubbles.
￭
Ensure that the connectors for the eluent
degasser are firmly connected .
￭
Deaerate the high-pressure pump (see Fig-
ure , page 55).
High-pressure pump –
Defective.
Request Metrohm Service.
Peak areas are
lower than
expected.
Sample – There is a leak in
the sample path.
Find and fix the leak in the sample path.
Sample – The sample path
is blocked.
Find and fix the blockage in the sample path.
Sample – The sample loop
is not (completely) filled.
Adjust the transfer time for the sample (see
"Determining the transfer time", page 99).
Sample – There are gas
bubbles in the sample.
Use the sample degasser .
MCS – The CO
2
suppressor
is not connected.
Connect the CO
2
suppressor.
The peristaltic pump
is pumping too little.
Peristaltic pump – Contact
pressure too weak.
Correctly set the contact pressure .
Peristaltic pump – Filter
blocked.