Metrohm 940 Professional IC Vario ONE/SeS/PP/Prep 2 User Manual

Page 71

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3 Installation

940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)

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61

2 Installing the input of the separation column

There are 3 possibilities:

Attaching the bottom end of the separation column directly to
the guard column.
or

If the guard column is connected to the separation column using
a provided connection capillary, connect the bottom end of the
separation column to the guard column's output capillary using
the provided PEEK pressure screw (6.2744.070).
or

If no guard column is used (not recommended), connect the col-
umn input capillary to the input of the separation column using a
short pressure screw (6.2744.070).

3 Rinsing the separation column

Place a beaker under the separation column's outlet.

Start manual control in MagIC Net and select the high-pressure
pump: Manual

Manual control Pump

Flow: in accordance with column leaflet
On

Rinse the separation column with eluent for approximately 10
minutes.

Use manual control in MagIC Net to stop the high-pressure pump
again: Off.

4 Removing the coupling

Remove the coupling (6.2744.040) from the column output capil-
lary.

5 Installing the output of the separation column

Fasten the column output capillary to the upper end of the sepa-
ration column using a PEEK pressure screw (6.2744.070).

6 Inserting the separation column

Insert the separation column with chip into the column holder
until you hear it snap in place.

The separation column is now detected by MagIC Net.

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