Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 17)

It is recommended that this function be performed a second time prior to first use to remove
any air that might have entered the system. The system has now been flushed with ethanol
and water, and is ready for use.
2.2.3 Setting the Local Time and Date
During purification runs, sample elution time is shown according to the system clock time
as set by the user. To set the system time and date:
1. Touch Data/Utilities from the system home screen.
2. Touch the Diag/Maint Functions button.
3. Touch Time and Date Setup.
4. Touch the arrows adjacent date and time settings to adjust as desired (Figure 2.8);
touch OK when finished to retain new settings and return to the Diag/Maint Functions
screen.
Fig. 2.8. Set Time & Date screen.
2.2.4 Validating the System
Performing an initial validation run of the Profinia system is recommended. This will allow
you to familiarize yourself with the set up, operation, and expected results of a purification
run. Use the starter kit provided with the instrument; if you need a new starter kit or
additional Profinia control lysate, see Appendix B for ordering information.
The starter kit includes a His/GST dual-tag control protein lysate to be used for this
validation run. The results are characterized in this section. The dual-tag control lysate can
be used with both the native IMAC and GST purification methods. To prepare and use the
control lysate:
1. Prepare the sample lysate by reconstituting it with 12 ml of 1x IMAC or GST lysis buffer
(dilute 6 ml of IMAC or GST lysis buffer 1:1 with 6 ml of deionized water to make 12 ml
of a 1x solution). This yields enough lysate for two of the 1 ml affinity runs.
2. Transfer 6 ml of resuspended sample to a 15 ml conical tube.
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