Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 53)

Fig. 6.18. Installation of a tube into fraction tube position 1D.
When all fraction tubes required in the run have been installed, touch the Next button to
begin the cartridge installation procedure.
6.6 Installing Cartridges
The cartridge installation process includes preparatory steps such as priming the system
cartridge lines to prevent introduction of air bubbles into the cartridge. The cartridge
installation routine is carried out through four consecutive screens:
• Select Cartridge Status — includes the option to bypass the installation
procedure when cartridges are already installed (for example, from a previous run)
• Setup Cartridge Positions for Priming — directs connection of the cartridge 1 and 2
position fittings
• Priming in Progress — displays the priming time progress bar
• Install Cartridges — displays the required cartridges and their respective positions
Warning: When the desalting-only method is selected DO NOT fill the sample loop with
sample until the sample loop and cartridge installation is completed. Inject the sample
immediately prior to starting the run. (See Section 6.6.5 for sample loop installation instructions.)
6.6.1 Select Cartridge Status
Two options are available on the Prepare for Cartridge Installation screen (Figure 6.19):
• No — cartridges are not installed and the system cartridge lines need to be primed;
touch the No button to proceed to the Prepare for Cartridge Line Priming screen
• Yes — cartridges are already installed and priming is not needed; touch the Yes
button to bypass priming and proceed directly to the Start Run screen (Section 6.7)
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