Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 31)

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2B. Waste and Water Bottles
1. Empty the waste container, and check the diluent
water bottle for sufficient volume.
2C. Sample Tube Installation
1. Add sample to 15 ml or 50 ml conical tubes.
2. Place sample tube lids on the conical tubes.
3. Lift the sipper tube away from the instrument.
4. Insert the conical tube for each position.
2D. Fraction Tube Installation
1. Insert bottom of tube into the fraction tube
holder indent.
2. Tilt the top of the tube into the top holder and
push into place.
Numbering of Collection Tubes
Sample 1 Sample 2
Flow-through 1A 2A
Wash – 1 1B 2B
Wash – 2 1C 2C
Purified protein 1D 2D
Correct installation of
conical tube in the
sample 1 position.
Water volume
required for
selected run
Correct installation of fraction collection tubes in
positions 1A through 1D. Setup shown is for one
sample, as indicated on the display screen
diagram above.
2 1
Sample
sipper tube
Sample lid
on conical
tube