Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 59)

Fig. 6.26. Lower fitting tightened into place (left) and upper cartridge fitting tightened into place (right);
cartridge 2 position remains closed.
When cartridge installation is complete, touch the Next button in the lower right corner of
the screen. You will proceed to the Start Run screen.
6.6.5 Installing the Sample Loop (Desalting-Only Methods)
Warning: When the desalting-only method is selected, DO NOT fill the sample loop with
sample until the sample loop and cartridge installation is completed. Inject the sample
immediately prior to starting the run.
Sample loading for a desalting-only method is achieved via a sample loop. Installation is
directed by the Install Bio-Scale Mini Cartridges screen (Figure 6.27). The desalting sample
loop accessory connects to the instrument in the cartridge 1 position and the desalting
cartridge in the cartridge 2 position (Figure 6.29).
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