ANKOM 2000 Series - Manual (Page 22)

Operator’s Manual
pg. 22
Rev E 1/14/15
11. Press the arrow keys on the Keypad until you see “Select NDF” on the
Display.
Select NDF
 v <Enter>
IMPORTANT
The NDF analysis will use the following default settings:

75 minute digestion

four 5 minute rinses
If you want to run a custom NDF analysis that allows you to set the digestion time and the
number of rinses, press the arrow keys until you see “Select Custom” on the Display.
12. Press ENTER on the Keypad and follow the prompts on the Display to set up the instrument for NDF
analysis.
If you do not want to use the Cubetainers to automatically add your solutions, you
can manually fill the vessel for each procedure. First make sure that the hoses to
the Cubetainers are disconnected. Then press START on the Keypad and
immediately pour 2L of solution directly into the vessel. Please note that the
solution must cover the level sensor in order for the instrument to start its
operation.
13. Press START on the Keypad. Solution from the Cubetainer will flow into the vessel through Port A.
Once the analysis begins, digestions and rinses occur automatically, with the
analyzer Display providing information about the process time remaining, the
temperature, and the pressure. Pressing STOP on the Keypad at any time during
the analysis ends the operation and opens the drain to exhaust the solution.
14. After the ND solution has been automatically inserted and agitation begins, manually add 20g of Na
2
SO
3
and 4.0ml of alpha-amylase directly into the Vessel.
15. Close the Vessel Lid and tighten it by turning the Vessel Clamp Handle.
16. When the “Process Complete” message appears on the Display, the analyzer operation is complete.
Open the Vessel Lid and remove the Samples at this time.
17. Gently press out excess water from the bags.
18. Place bags in a 250ml beaker and add enough acetone to cover them. Let the bags soak in acetone for
3 – 5 minutes.
19. Remove the bags from the acetone and place them on a wire screen to air-dry.