Troubleshooting, Quality of the sample, Data not received – ChemoMetec SCC-100 User Manual

Page 35: Uality of the sample, Ata not received, 6 troubleshooting

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6 Troubleshooting

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6 Troubleshooting

Occasionally difficulties with the SomaticView software may occur. In the following a
short description and suggested action is given.

6.1

Quality of the sample

Through the CM file image it is possible visually to assess the quality of the sample
preparation and thereby the reliability of the measurement by looking at the “starry
sky”. The measurement is reliable if the stars or white dots are distributed separately
and evenly over the image. Naturally, this rule only applies when a representative
sample has been drawn from the original Milk sample.

Be aware of large objects in the image. These objects can be external material, such as
dust or a piece of hair. These objects are ignored in the image analysis so the obtained
result correctly reflects the number of individual cells in the sample. On the other hand,
it is important to be aware of these objects. If it is a cell aggregate or clumps, it may
indicate that the sample preparation has been sub optimal and the quality of the
estimate of cell concentration may be compromised.

6.2

Data not received

If SomaticView does not receive data from the NucleoCounter SCC-100, please verify
that the USB cable is connected correctly to both the NucleoCounter SCC-100 and the
computer. Then look at the task bar on the computer to see if the NC Receiver is started.

Figure

Figure

Figure

Figure 32

32

32

32 NC Receiver icon on the Windows task bar

The NC Receiver must be started to receive data from the NucleoCounter SCC-100. If the
NC Receiver is not open, quit and restart SomaticView. NC Receiver should then appear
on the task bar.

Generally the NC Receiver has two states in the Info text field when it is started:

• Input open failure

• Input opened

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