Using the light source – UVP 81-0333-01 BioLite Xe User Manual

Page 11

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BioLite Xe MultiSpectral Light Source

11

Using the Light Source

1. Set the power switch on the front of the system to the ON (I) position.

2. Use the VisionWorksLS “BioLite” module (shown below) to adjust the various BioLite settings:

a. Filter: Select the desired excitation filter position from the dropdown menu.

b. Light Engine: Turn the BioLite bulb on or off using the radio buttons (note that the

BioLite’s green power switch must be in the ON position to use the lighting controls).
When the bulb is on, the lower indicator light to the left of the switch on the front of the
BioLite will glow red.

NOTE:
For optimal BioLite Xe performance and operation, each time the xenon light is
turned off it is important to wait at least 30 seconds before turning it back on again.

c.

Intensity: This setting regulates the amount of illumination in six increments. The relative
output levels are specified in the chart below:

Intensity Setting

Relative Intensity

6

100%

5

50%

4

40%

3

25%

2

12%

1

0%


Light Table: Select between either Epi (overhead) or Trans (base lighting) depending on
the application. Note that both the epi and transillumination cannot be used
simultaneously.

NOTE: If using the darkroom to perform light-tight experiments such as capturing a
chemiluminescence sample for more than two minutes, use the black rubber cap to cover the end
of the fiber optic cable. Exposing an image for less than two minutes with the rubber cap removed
will not impact the resultant image, so long as the fiber optic cable is installed in the BioLite.
Without capping the fiber optic cable, light may enter the darkroom through the end of the fiber
optic cable, thus affecting the results of an extended resolution image.

3. It is possible to adjust the BioLite’s settings using the switches located on the front of the BioLite.

Note, however, that any settings modified using the switches will NOT be reflected in
VisionWorksLS’ BioLite software interface.

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