Hach-Lange Piston pipette - Manual (Page 18)

16
Using the pipette
2.3 Pipetting techniques
The pipette is operated by means of the push button at the
end of the handle.
Maximum accuracy can be achieved by carrying out the
following steps:
1. Move the piston up or down slowly and evenly. (especially
when pipetting highly viscous liquids)
2. Do not allow the push button to spring back into its original
position.
3. Use light pressure to fit a new tip to the cone, turning the
tip slightly as you do so.
4. Make sure that the tip is firmly in position on the tip cone
and there are no foreign bodies between the tip and the
cone.
5. Wet the tips with the liquid by repeatedly filling and
emptying them.
6. Hold the pipette almost vertical (no more than 10° from the
vertical) while liquid is being drawn into the tip.
7. The temperature of the pipette and the tip should match
that of the liquid.
AV_pipette.book Seite 16 Donnerstag, 8. Dezember 2005 3:56 15