Luminex 100 IS User Manual, Version 2.3 - Manual (Page 30)

Luminex 100 IS User Manual Version 2.3
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MAP Technology
3 - 4 PN 89-00002-00-071 Rev. C
acquiring sample, running system calibrators, running system
controls, and warming up the instrument, the system resets the
four-hour internal clock.
Accuracy and Precision
• Sample uptake volume: ± 5%
• Classification of xMAP microspheres: > 80%
• Misclassification of xMAP microspheres:
≤ 2% - may vary by
xMAP microsphere product lines. Refer to the specific product
information sheet for further details.
• Temperature control: 0°C to + 2°C of target
• Internal sample carry over: < 0.9%
• Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity values
Sensitivity
• Detect 1000 fluorochromes phycoerythrin (PE) per xMAP
microsphere
• Reporter channel dynamic range: 3.5 decades of detection
Capacity
The specifications below reflect minimum capacity values:
• 10 GB hard drive
• 100 MB read/write CD ROM
• Analyze multiple 96-well plates per batch
• Analyze multiple assay templates per plate
• Distinguish a minimum of 1 to a maximum of 100 unique xMAP
microsphere sets in a single sample
• Detect and distinguish surface reporter fluorescence emissions at
575 nm on the surface of 1-100 unique xMAP microspheres sets
in a single sample
• Sample core: 15-20 µm core at 1 µL/sec. sample inject rate
• Maintain samples at a constant temperature from 35°C to 55°C
(95°F to 131°F)
• Automatic sampling from a 96-well plate
• Start sampling from any well position
• Sheath container and waste container hold enough volume to run
up to two 96-well plates between refills
• Microtiter plates with 96 wells must be compatible with the
Luminex XYP instrument plate holder. The following microtiter
plate types are compatible with the Luminex XYP instrument
plate holder: flatbottom, conical, round, filter bottom, half plates
[overall height no more than 0.75” (19 mm)], any color
• Microtiter plates with 96 wells must be compatible with
Luminex XYP instrument heater block temperature from 35°C to