Multichannel Systems Roboocyte2 Manual - Manual (Page 32)

Roboocyte2 Manual
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Now the voltage trace should exactly run at -60 mV and the holding current should be close
to -600 nA (because the "oocyte membrane resistance" of the TMC is equal to 100 kOhm).
If you switch to different holding potentials during the recording, the voltage trace should
be following immediately together with a change in holding current.
Voltage-Step Protocol
In order to test the step-response of the built-in amplifier it is best to perform a voltage-step
protocol with the test-model cell. For this purpose, there is a pre-defined voltage-step protocol
called "TMC-test". First, load the "TMC-test" voltage protocol into the program. Press the "Load"
button in the "Voltage Step Protocol" section and select "TMC test".
First, load the "TMC-test" voltage protocol into the program. Press the "Load" button in the
"Voltage Step Protocol" section and select "TMC test".
Press "Apply" and the voltage-step protocol will be executed. The data display will now show
the following.