Multichannel Systems Roboocyte2 Manual - Manual (Page 83)

Recording with Roboocyte2 Control Software
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If the impalement was successful, you will notice a change in voltage on both electrodes,
for example the membrane potential of the oocyte is recorded.
5. If the membrane potential of the oocyte is not detected on one or both electrodes, move the
measuring head stepwise down using the arrow keys. Stop moving the measuring head as soon
as you record the membrane potential on both electrodes.
Both glass microelectrodes record the oocyte membrane potential after proper impalement.
Now, after checking the electrode resistances, performing offset correction and stability check,
as well as successfully impaling the cell, you are ready to start the recording under voltage clamp.