8 analysis with roboocyte2, 1 the roboocyte2+ analysis software – Multichannel Systems Roboocyte2 Manual User Manual

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8 Analysis

with

Roboocyte2+

8.1 The Roboocyte2+ Analysis Software

8.1.1 Analysis

with

Roboocyte2+

The Roboocyte2+ program is the offline analyzer part of the Roboocyte2 software package. It is
intended for analysis and review of the data, for ASCII export, and for visualization of the results.
The Roboocyte2+ program uses both information stored in the database used for recording and
information stored in the plate files. Before loading a plate file, the corresponding database has
to be loaded. When you close the program the database will be used to save the changes you
made to your analysis.

Note: Under no circumstances, the use of any program feature will modify your original data.

Main features of the program are:

 Review recordings.

 Extract current or voltage values from your recorded traces.

 Moving ROIs (regions of interest), leak cursors and baseline cursors from positions defined

in the recording script.

 Performing P/n leak subtraction. This feature is accessible if the leak pulses have been recorded

during the experiment.

 Performing baseline and drift correction.

 Generate IV dependencies.

 Generate and fit dose-response curves.

 Exporting raw traces, IV relationships and dose-response curves (with fit parameters) in ASCII

format.

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