Eppendorf AF2200 Plate Reader - Manual (Page 53)

Maintenance
Eppendorf ® PlateReader AF2200
English (EN)
53
Pipette 200 μL of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells
according to the plate layout below.
Material/reagents
• 1 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)
• 0.01 M NaOH (=blank solution) (NaOH pellets)
• 96-well plate
• 200 μL pipette with tips
Calculating the precision within a plate
The results of the formula specifies the precision within a plate in % CV (coefficient of variation).
X 1 nM fluorescein
blank 0.01 M NaOH
Mean value
F
Mean RFU value of the wells filled with fluorophore
Std. deviation
F
Standard deviation of the RFU values of the wells
filled with fluorophore
F
F
mean
100 * stdev
(%) Uniformity =