Eppendorf AF2200 Plate Reader - Manual (Page 54)

Maintenance
Eppendorf
® PlateReader AF2200
English (EN)
54
Precision from one measurement to the next
Carry out the following measurement to determine the precision and reproducibility.
Pipette 200 μL of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells
according to the plate layout below.
Material/reagents
• 1 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)
• 0.01 M NaOH (=blank solution) (NaOH pellets)
• 96-well plate
• 200 μL pipette with tips
Parameter Setting
Measuring mode Upper fluorescence
Wavelength, excitation 485 (20 nm)
Wavelength, emission 535 (25 nm)
Number of flashes 25
Integration time 40 (μs)
Setting time 0 (μs)
Signal gain Optimal
Plate type GRE96fb
Part of plate A1
Kinetic 20 cycles
Time interval Minimum
X 1 nM fluorescein
blank 0.01 M NaOH