Eppendorf AF2200 Plate Reader - Manual (Page 57)

Maintenance
Eppendorf ® PlateReader AF2200
English (EN)
57
Precision across the plate
Carry out the following measurement to determine the precision across the plate.
Pipette 200 μL of 25 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding wells
according to the plate layout below.
Material/reagents
• 25 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)
• 0.01 M NaOH (=blank solution) (NaOH pellets)
• 96-well plate
• 200 μL pipette with tips
Parameter Setting
Measuring mode Lower fluorescence
Wavelength, excitation 485 (20 nm)
Wavelength, emission 535 (25 nm)
Number of flashes 25
Integration time 40 (μs)
Setting time 0 (μs)
Signal gain Optimal
Plate type GRE96fb
X 25 nM fluorescein
blank 0.01 M NaOH