Eppendorf BioSpectrometer kinetic - Manual (Page 30)

Methods
Eppendorf BioSpectrometer
® kinetic
English (EN)
30
You can create new methods in all folders using <New Method>.
In Favorites, you can create your own folders (e.g., to allocate folders to specific people), and rename and
delete the folders.
Tab. 6-2: Softkeys in method selection
Copied or cut methods can be added to a different folder under Favorites, or added to the original folder
under a new name. Use the cursor keys to navigate to the
Methods column of the desired folder and press
[paste] for adding the method.
6.2 Photometry method description
The preprogrammed methods and method templates are described in this section.
6.2.1 Absorbance method group
Single λ
• Absorbance measurement on a wavelength.
• No subsequent evaluation.
Single λ - continuous
• Repeated absorbance measurement on a wavelength.
• Parameters can be entered for temperature control between 20 and 42°C (presetting: 37°C).
• Entry of parameters for total time and interval time for the measuring points. A premature stop cannot
be made during the measurement.
• Evaluation as kinetic via linear regression. A subsequent change cannot be made to the time frame for
the evaluation.
• The measured values are displayed in an absorbance time graph.
• To subsequently evaluate measured values as linear regression kinetics, press the [Next >] softkey and
go to the process results method step.
Multi λ
• Absorbance measurement at two to six wavelengths.
• No subsequent evaluation.
Scan
• Absorbance wavelength spectra measurement via a defined wavelength range.
• Display of wavelength and absorbance in the spectrum by navigation with a wavelength cursor.
• The spectra section can be modified using 3 different zoom variants.
• Peak detection possible.
[Cut] and [Paste] Cut and paste methods.
[Copy] and [Paste] Copy and paste methods.
[Delete] Delete methods.
[Rename] Rename methods.