Eppendorf Mastercycler pro - Manual (Page 50)

PCR run
Mastercycler pro
English (EN)
50
7.1.2 Loading the thermoblock
7.1.2.1 Loading a 96 well block
The Mastercycler pro and Mastercycler pro S can be loaded with 0.2 mL single tubes or strips. You can also
use a 96-well PCR plate. The following PCR plate types are compatible with the Mastercycler pro:
7.1.2.2 Loading a 384-well block
The Mastercycler pro 384 can be loaded with a 384-well PCR plate.
To seal the PCR plates, you can use adhesive and heat sealing films, lid strips or reusable silicone mats.
A selection of the PCR micro test tubes and lids can be found separately (see Consumables on p. 86).
7.1.3 Sample volume
The liquid level in the micro test tube should not be above the upper edge of the block.
You can use the following standard values for the maximum sample volumes:
• Mastercycler pro and Mastercycler pro S: Maximum 100 μL per single reaction.
• Mastercycler pro 384: Maximum 25 μL per single reaction.
The sample volumes that can actually be used may vary depending on the type of micro test tube used.
CAUTION! Damage to the thermoblock from incorrect operation.
 Do not fill sample material directly into the thermoblock.
Semi-skirted PCR plate Skirted PCR plate Non-skirted/unskirted PCR plate
PCR plate with surrounding
half-edge. PCR plate with a surrounding
edge. PCR plate without any surrounding
edge.