3 interrupting or stopping the program, 1 interrupting the program, 2 continuing the program – Eppendorf Mastercycler pro User Manual

Page 53: 3 stopping the program, 4 using protocols, Interrupting or stopping the program 7.3.1, Interrupting the program, Continuing the program, Stopping the program, Using protocols

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PCR run

Mastercycler pro

English (EN)

53

7.3

Interrupting or stopping the program

You can temporarily interrupt the program run in the status view, for instance, to add reagent, or
completely stop the program.

7.3.1

Interrupting the program

1. Open status view (see Starting the program on p. 51).

2. Press the Stop key.

The status view shows

Paused

.

Observe the safety precautions for opening the heated lid (see Opening the heated lid on p. 28).

7.3.2

Continuing the program

1. If the heated lid is opened, draw the heated lid forwards over the samples and close the lid.

2. Press the

Resume

function key.

The status view displays

running

and the program run is continued.

7.3.3

Stopping the program

 Press the

Abort

function key.

The status

Idle

is displayed. The program has been aborted and cannot be continued.

7.4

Using protocols

The Mastercycler pro creates a protocol for each program run where all executed program steps and any
interruptions and errors are recorded.

After running a PCR program, you can view the associated protocol on the control panel, save it on an MMC
or a USB memory or send it to a printer.

Prerequisites

The cycler is idling.

1. Mark the desired cycler node in the navigation tree.

2. Press the function key

Protocol

.

The protocol is overwritten the next time a program is started on the Mastercycler pro.

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