Leica Biosystems SM2000R - Manual (Page 14)

Instruction manual V2.1 – 03/2004 14
7. Operating the instrument
7.1 Mounting and orienting the specimen
Prior to manipulating the knife or specimen,
or changing the specimen or knife, and dur-
ing breaks, always cover the cutting edge
with knife guard and lock the knife sledge
with the locking knob!
• Clamp an embedding cassette in the universal cas-
sette clamp (6) or a paraffin block in the standard
specimen clamp (7).
• To orient the specimen surface towards the knife,
loosen the locking lever (8).
• Orient the sample by means of the adjusting screws
(9) and (10).
• Retighten the locking lever (8) after orienting.
7.2 Clearance angle adjustment on the knife
holder
• Loosen the clamping screw (19) and the knife
clamping screw (16).
• Select the desired angle with the adjusting lever (20)
on the clearance angle scale (21).
• Retighten the clamping screws (19) and (16) to fix
the angle setting.
7.3 Inserting the knife or disposable blade
holder
Take care when handling microtome knives
and disposable blades!
The cutting edge is extremely sharp and may
cause severe injury!
• Unscrew the knife clamping screw (16) on the knife
holder.
• Carefully insert the knife or disposable blade holder
(15, Fig. 14.1) with the blade into the knife holder
from the left and hold in place.
• To clamp turn the knife clamping screw (16) clock-
wise.
The screw does not need to be fully tightened. Good
sectioning results may be obtained when the screw
is tightened with a medium effort.
Fig. 14.2
Fig. 14.1
20
19
15
6
9
8
10
16
14
21
16