Leica Biosystems SM2000R - Manual (Page 16)

Instruction manual V2.1 – 03/2004 16
8. Trouble shooting
Systematically try several clearance
angle adjustments, until the optimum
angle is found.
- Check if all levers are locked and
screws are tightened on the speci-
men and knife holder systems. Re-
tighten the levers and screws if nec-
essary.
- Use a different part of the knife or
blade edge or use a new knife/blade.
- Use a different part of the knife or
blade edge or use a new knife/blade.
- Precool the specimen on a cold
plate.
- Cool the specimen in iced water im-
mediately before sectioning.
- Systematically decrease the clear-
ance angle until the optimum adjust-
ment is obtained.
- Systematically decrease the clear-
ance angle until the optimum adjust-
ment is obtained.
- Use knife with different profile.
- Check if all levers are locked and
screws are tightened on the speci-
men and knife holder systems. Re-
tighten the levers and screws if nec-
essary.
- Turn the coarse feed wheel clock-
wise to lower the specimen.
- Insufficient knife/blade inclination;
consequently the clearance angle is
too small.
- Insufficient clamping of specimen
and/or knife.
- Blunt knife/blade.
- Blunt knife/blade.
- Specimen too warm.
- Clearance angle too big.
- Clearance angle too big.
- Inappropriate knife profile.
- Insufficient clamping of specimen
and/or knife.
- Upper feed limit reached.
Thick/thin sections
The section thickness varies from one
section to another. In extreme cases,
sections are skipped meaning that a
section is not obtained.
Compressed sections
The sections are extremely com-
pressed, wrinkled or jammed together.
Sections exhibit scratches and chatter
marks.
No specimen feed takes place. No sec-
tion obtained.
Possible cause Problem Correction