Leica Biosystems SM2000R - Manual (Page 15)

15 Leica SM2000 R – Sliding Microtome
7. Operating the instrument
7.4 Trimming
For trimming, the specimen feed can be disengaged
either by turning the coarse feed wheel (2) or by oper-
ating the manual feed lever (3).
• Hold the knife sledge (11) at the grip (12) and place
the sledge behind the specimen.
• Pull the knife guard (14) of the knife holder to the
right.
• To feed the specimen towards the knife, turn the
coarse feed wheel (2) counterclockwise;
or
select the required section thickness with the sec-
tion thickness adjusting knob (1) and pull the manual
feed lever to the front (3). Each lever activation
causes a specimen feed by the selected value. The
coarse feed wheel will turn at the same time.
• Move the knife sledge forth and back until the speci-
men surface is trimmed as required.
7.5 Sectioning
• Select the required section thickness with the sec-
tion thickness adjusting knob (1, Fig. 15.1).
When using the automatic advance feature,
make sure to move the knife sledge against
the limit stop position to disengage an auto-
matic feed after each section.
The position of the adjusting knob (22) determines the
point where the automatic feed takes place. The feed
should take place immediately before the specimen,
or behind the sample from the operator‘s view.
• To use the automatic feed feature, loosen the ad-
justing knob (22), place it in the required position,
and retighten the knob.
• To produce the section, pull the knife sledge over
the specimen at a constant speed.
Caution! The knife sledge movement is ex-
tremely smooth. It may occur that the sledge
moves during section removal.
To prevent the sledge from moving acciden-
tally, pull it to the front limit of the slideway,
where it is held in position by the magnetic
knife sledge immobilizer (24), before remov-
ing the section.
• Carefully remove the section with a small brush.
24
22
Fig. 15.1
Fig. 15.2
14
11
1
2
3