Metrohm 881 Compact IC pro – Cation - Manual (Page 53)

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3 Installation
881 Compact IC pro – Cation
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in the Internet at http://www.metrohm.com in the Applications area or via
the Metrohm agent responsible free of charge.
Note
The separation column may only be installed after the initial start-up
(see Chapter 4.1, page 47) of the instrument. Until then, use the cou-
pling (6.2744.040) instead of the guard and separation column.
Connecting and rinsing the separation column
1 Connect the separation column
Caution
When inserting the columns, always ensure that these are cor-
rectly inserted corresponding to the flow direction indicated.
￭ Remove stoppers from the separation column.
￭ Attach the guard column to the input of the separation column.
OR
Connect the input of the separation column to the outlet capillary
of the guard column, using the PEEK pressure screw (6.2744.070)
included.
OR
If no guard column is used (not recommended), connect the col-
umn input capillary (3-2) to the input of the separation column,
using a PEEK pressure screw (6.2744.070).
2 Rinsing the separation column
￭ Place beaker under the outlet end of the separation column.
￭ Set the flow rate of the high pressure pump according to the data
given in the leaflet of the separation column.
￭ Start the high pressure pump and rinse the separation column
approx. 10 minutes with eluent.
￭ Switch off the high pressure pump again.
3 Mounting the separation column
￭ Fasten the column output capillary to the output of the separation
column using a PEEK pressure screw (6.2744.070).
￭ Hang separation column with chip into the column holder.