2 connect drainage tube, 3 connecting the pulsation absorber, 4 connection of the sample path – Metrohm 871 Advanced Bioscan User Manual

Page 21: Connect drainage tube, Connecting the pulsation absorber, Connection of the sample path, Ction 2.4.4

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2.4 Capillary connections

871 Advanced Bioscan / Instructions for Use 8.871.1003

13

2.4.2

Connect Drainage tube

Connect silicone tube 6.1816.050 to the drain opening 3 and lead it to a

waste container.

2.4.3

Connecting the pulsation absorber

The 818 IC Pump needs to be deaerated before putting it into opera-

he 818 IC Pump Instructions for

Use.

tion. The procedure is described in t

To protect the column material from any pressure shocks which the in-

jection may cause we recommend the insertion of the 6.2620.150 MF

Pulsation absorber between the high-pressure pump and the injec-

ollows (see Figure 3):

tion valve. This is done as f

1 Connect the pulsation absorber

•

Place pulsation absorber 26 on the floor in the interior of the

871 Advanced Bioscan.

2 Connection to pump

•

Connect the 6.2821.120 PEEK Filter unit described in the

r unit

outwards through opening 2 of the Bioscan and attach it to

connections on the top side of the pulsation

Instructions for Use 818 IC Pump to the 818 IC Pump.

•

Lead PEEK capillary 25 from connection piece of the filte

one of the 26

absorber.

3 Connection to injection valve

•

Connect PEEK capillary 27 to the second connection of the

pulsation absorber 26 and to the injection valve 18 (see

Figure 3).

The pu

with eluent

before a separating column is connected. To do that, dis-

connec

aste

containe

lsation absorber is filled with isopropanol and must be rinsed

t PEEK capillary

27

from the injection valve, lead it into a w

r an turn on the 818 IC pump for 10 min.

20.150 Pulsation absorber can be operated in both direc-

f the sample path

ction of the sample path is done as follows (see Figure 3):

ct aspirating tube

illary 24 through opening 2 of the Advanced

nnect it to the injection valve 18.

The 6.26

tions.

2.4.4

Connection o

Conne

1 Conne

•

Lead PEEK cap

Bioscan and co

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