Metrohm 850 Professional IC Cation - Prep 2 - Manual (Page 70)

2.20 Separation column
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850 Professional IC – Cation – Prep 2
Connecting and rinsing the separation column
1 Connecting the separation column
Caution
When using the columns, always ensure that these are correctly
inserted corresponding to the flow direction indicated.
￭ Remove stoppers from the separation column.
￭ Connect the lower end of the separation column with a
6.2744.070 short PEEK pressure screw to the outlet capillary of
the guard column (if used), or to the column input capillary .
2 Rinsing the separation column
￭ Place beaker under the outlet end of the separation column.
￭ Set the flow rate of the high pressure pump to the value suitable
for the separation column selected.
￭ Start the high pressure pump and rinse the separation column
approx. 10 minutes with eluent.
￭ Switch off the high pressure pump again.
3 Mounting the separation column
￭ Hang separation column with chip into the column holder.
Note
The iColumns are equipped with a chip on which their operating data is
saved. The chip has to be hooked into the chip holder provided for this
so that the column recognition can function.