Metrohm 887 Professional UV/VIS Detector - Manual (Page 40)

6.6 Cleaning the flow-through cell ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
32 ￭￭￭￭￭￭￭￭ 887 Professional UV/VIS Detector
Rinsing the flow-through cell with methanol
1 Disconnect the inlet capillary from the IC system.
2 Connect the inlet capillary to a high pressure pump or a peristaltic
pump and rinse the flow-through cell as follows – ensure that the
maximum pressure of 5 MPa is not exceeded:
￭ first, rinse with ultrapure water to avoid precipitation,
￭ then, rinse several minutes with methanol, to dissolve the depos-
its, and
￭ finally, rinse another 15 minutes with ultrapure water, to wash
away the dissolved deposits.
3 Observe the baseline during the last rinsing step.
If the baseline remains flat, the flow-through cell is clean.
If the noise persists, rinse the flow-through cell once again with
another solvent (see "Rinsing the flow-through cell with another sol-
vent", page 32).
Rinsing the flow-through cell with another solvent
A solvent made up of a 1:2 mixture of acetic acid and isopropanol has
shown good results. Depending on the application, other solvents may
also prove efficient.
Preconditions:
￭ Rinsing with methanol was inefficient.
￭ The inlet capillary is connected to a high pressure pump or a peristaltic
pump.
1 Rinse the flow-through cell as follows – ensure that the maximum
pressure of 5MPa is not exceeded:
￭ first, rinse with ultrapure water to avoid precipitation,
￭ then, rinse several minutes with the selected solvent to dissolve
the deposits, and
￭ finally, rinse another 15 minutes with ultrapure water to wash
away the dissolved deposits.
2 Observe the baseline during the last rinsing step.
If the baseline remains flat, the flow-through cell is clean.