Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 28)

Section 10
Detection
10.1 SDS-PAGE and Native PAGE Detection
Total Protein Gel Stains
Method Sensitivity Optimal Protein Load Advantages Disadvantages
Coomassie Blue R-250 36–47 ng ~0.5 µg/band Laboratory standard Requires MeOH
Bio-Safe™ Coomassie stain 8–28 ng ~0.5 µg/band Nonhazardous, uses More steps than
no MeOH Coomassie Blue R-250
Copper stain 6–12 ng ~0.2 µg/band Fast, reversible stain Negative stain, must
be photographed;
SDS-PAGE only
Zinc stain 6–12 ng ~0.2 µg/band High-contrast, fast, Negative stain, must
reversible stain be photographed;
SDS-PAGE only
Silver Stain™ Plus kit 0.6–1.2 ng ~0.01 µg/band Simple, robust, mass Will not stain
spectrometry compatible glycoproteins
Silver stain 0.6–1.2 ng ~0.01 µg/band Stains complex Not mass spectrometry
proteins, i.e., glycoproteins compatible
and lipoproteins
SYPRO Orange protein 4–8 ng ~0.2 µg/band Will not stain nucleic Optimization required
stain acids; mass spectrometry for maximum
compatible sensitivity
SYPRO Ruby protein gel 1–10 ng ~0.2 µg/band Broad dynamic range, Requires imaging
stain simple robust protocol instrument for
maximum sensitivity
Flamingo Fluorescent 0.25–0.5 ng ~0,2 ng/band Broad dynamic range Requires imaging instrument
gel stain mass spec compatible for maximum sensitivity
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