Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 31)

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10.3 IEF Gel Staining
Samples on IEF gels can be detected using multiple methods. Use the following table as a guide to select
an appropriate staining method.
Method Sensitivity Optimal Protein Load Advantages Disadvantages
IEF stain 40–50 ng ~0.5 µg/band Simple, no fixation required Requires MeOH
Silver Stain Plus kit 0.6–1.2 ng ~0.01 µg/band Simple, robust, mass Requires TCA
spectrometry compatible fixation
Silver stain 0.6–1.2 ng ~0.01 µg/band Stains complex Requires TCA
proteins, i.e. glycoproteins fixation
and lipoproteins
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