Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 36)

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IEF Destaining Solution
Final Concentration
Methanol 400 ml 40%
Acetic acid 100 ml 10%
Deionized water 500 ml
11.3 Peptide Staining Solutions
Fixative Solution
Final Concentration
Methanol 400 ml 40%
Acetic acid 100 ml 10%
Deionized water 500 ml
Coomassie Blue G-250 Staining Solution (0.025%)
Final Concentration
Acetic acid 100 ml 10%
Coomassie Blue G-250 0.25 g 0.025%
Deionized water 900 ml
Coomassie Blue G-250 Destaining Solution
Final Concentration
Acetic acid 100 ml 10%
Deionized water 900 ml
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