Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis User Manual
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Section 8 Nondenaturing Nucleic Acid PAGE...................................................................... 17
8.1 Introduction......................................................................................................................................... 17
8.2 Ready Gel TBE Gel Composition.........................................................................................................17
8.3 Ready Gel TBE Gel Selection Guide.................................................................................................... 17
8.4 Nondenaturing Nucleic Acid PAGE Buffers.......................................................................................... 18
8.5 Sample Preparation............................................................................................................................. 18
8.6 Running Conditions............................................................................................................................. 18
Section 9 Denaturing Nucleic Acid PAGE............................................................................19
9.1 Introduction......................................................................................................................................... 19
9.2 Ready Gel TBE-Urea Gel Composition................................................................................................ 19
9.3 Ready Gel TBE-Urea Gel Selection Guide........................................................................................... 19
9.4 TBE-Urea Buffers................................................................................................................................ 20
9.5 Sample Preparation............................................................................................................................ 20
9.6 Running Conditions............................................................................................................................ 20
Section 10 Detection.......................................................................................................... 21
10.1 SDS-PAGE and Native PAGE Detection...................................................................................... 21–22
10.2 Peptide Gel Staining......................................................................................................................... 23
10.3 IEF Gel Staining................................................................................................................................ 24
10.4 Zymogram Gel Staining.................................................................................................................... 25
10.5 TBE Gel Staining.............................................................................................................................. 26
10.6 TBE-Urea Gel Staining...................................................................................................................... 26
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