Bio-Rad Ready Gel Precast Gels for 2-D Electrophoresis - Manual (Page 40)

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Selectively remove predominant protein in
the sample
Sample precipitation Centrifuge samples to remove particulates
prior to sample loading
Gels run too fast, provide poor resolution, Running buffer is too concentrated Check buffer composition
and gel temperature is too high
Artifact bands at ~60–70 kD Possible skin keratin contamination Clean all dishware and wear gloves
while handling and loading gel
Filter all solutions through nitrocellulose
Use 10% iodoacetamide to eliminate
keratin bands
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