Bio-Rad Clarity™ Western ECL Substrate - Manual (Page 8)

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Fig. 2
Chemiluminescence
detection by HRP.
The secondary antibody
is linked to an enzyme,
which catalyzes a reaction
leading to light emission.
Luminol oxidized by
HRP in the presence
of a peroxide leads to
the formulation of a
3-aminophthalate dianion
and the release of light.