Bio-Rad Model 785 Vacuum Blotter - Manual (Page 12)

3.5 Post-Transfer
1. After 90 minutes, turn off the vacuum source.
2. Squeeze the Sealing Frame spring handle inward to release it from the latch posts.
Remove the Sealing Frame. Let the upper buffer drain into the Base Unit.
3. Remove the transferred gel. The gel area inside the window should be half as thick as
the gel area outside the window. To check for any remaining DNA, stain the gel with
1.0 µg/ml of Ethidium Bromide.
4. Remove Window Gasket and nylon membrane. Soak the membrane in 2x SSC for
5 minutes and air-dry between two sheets of filter paper. Dry the membrane
between two sheets of filter paper in a vacuum oven at 80 degrees for 30 minutes.
The blotted membrane is now ready for hybridization.
5. Remove the filter paper on the Porous Vacuum Plate and dump the transfer
solution from the Base Unit. Clean the Model 785 Vacuum Blotter according to
Section 4.
Section 4
Instrument Maintenance
4.1 Cleaning
1. Rinse all Vacuum Blotter components with warm water. Remove any salts by rinsing
the Vacuum Stage and the Reservoir Seal O-ring with water. Clear all vacuum tubing
of any liquids before transferring.
Caution: Do NOT rinse or wet the Vacuum Regulator or the Vacuum Pump.
2. Remove any agarose particles or filter paper fiber from the Porous Vacuum Plate by
rinsing it with a strong jet of water. Be careful not to smash any agarose into the
Porous Vacuum Plate. These cleaning instructions will prevent the fine pores of the
Porous Vacuum Plate from clotting due to salts, filter paper fibers, or agarose.
4.2 Replacement Parts
The parts listed below will wear out over time. These parts have a definite lifetime,
depending on use and maintenance. Please examine these parts carefully before each use.
Replace these parts as needed:
Catalog
Number
Product Description
165-5007 Reservoir Seal O-ring, 1
165-5008 Blank Window Gasket, 6
165-5012 Porous Vacuum Plate, 1
8