Bio-Rad Model 785 Vacuum Blotter - Manual (Page 19)

Chart 3: Gel Concentration Effect On Transferring Efficiency
Vacuum Pressure - 5 inches Hg; Time of Transfer - 90 minutes; Gel percentage – varied.
7.2 Required Solutions and Equipment
The check list below consists of stock solutions and equipment that are necessary for
the vacuum transfer process.
A. Stock Solutions: To Make 1,000 ml Solution
1. 20x SSC 175.3 g of NaCl + 88.2 g of sodium citrate in 800 ml
of double distilled water. Adjust to pH 7.0 with a few
drops of concentrated HCl. Adjust to 1,000 ml.
2. 5 M NaCl 292.2 g of NaCl in 800 ml of double distilled water.
Adjust to 1,000 ml.
3. 5 N NaOH 200 g NaOH pellets in 800 ml of double distilled
water. Adjust to 1,000 ml. Caution: Highly
exothermic reaction.
4. HCl Purchase concentrated liquid stock.
100
80
60
40
20
0
0.8 kb 4.1 kb 15 kb 23 kb
F ragment Size
0.7% G el
1.0% G el
1.5% G el
%
o
f
T
ra
n
sf
e
r
15