Bio-Rad Model 785 Vacuum Blotter - Manual (Page 8)

2.5 Sealing Frame
The Sealing Frame is made of the same mindel-S plastic as the Base Unit and has the
same off-white color as the Base Unit. The Sealing Frame features easy-to-use latches.
The latches will lock on to the post when the Sealing Frame is pressed onto the Vacuum
Stage. Simply press the latches inward to unlock the Sealing Frame. The Sealing Frame
compresses the Reservoir Seal O-ring onto the Window Gasket, creating an air-tight seal
between the Porous Vacuum Plate and the Window Gasket. The Sealing Frame also
holds the transfer solution over the gel during the transfer.
2.6 Vacuum Regulator
The Vacuum Regulator is used to control the vacuum pressure. There is a post on
either end of the Vacuum Regulator. One post is used for the vacuum source inlet and the
other post is used to connect the Model 785 Vacuum Blotter. The Vacuum Regulator has
a vacuum gauge attached to the center portion. Because the vacuum gauge is sensitive to
liquids, it is very important to connect a side-arm flask between the Vacuum Blotter and
the Vacuum Regulator. There is an adjustment valve in front of the pressure gauge.
Unscrewing the outer-most adjustment nut causes the vacuum pressure to decrease.
Screwing the adjustment nut clockwise increases the pressure.
2.7 Vacuum Pump
The Vacuum Pump is used to create a vacuum underneath the gel/Window Gasket
complex. The Vacuum Pump is connected directly to the Vacuum Regulator with vacuum
tubing. The Vacuum Pump is either 100–120 VAC or 220–240 VAC. The voltage is not
user settable and is configured at the factory. Verify that the voltage of the pump is
appropriate for your country.
2.8 Lid
The Vacuum Blotter Lid is made of a clear polycarbonate. The Lid shields the gel
from falling objects during transfer. It also protects the instrument during storage. The
Model 785 Vacuum Blotter Lid allows you to stack vacuum blotting units on top of each
other without damaging the units.
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