Bio-Rad Model 785 Vacuum Blotter - Manual (Page 18)

Chart 1: Time Effect on Transferring Efficiency
Vacuum Pressure - 5 inches Hg.; Time of Transfer - varied; Gel Percentage - 0.7%.
Chart 2: Vacuum Effect on Transferring Efficiency
Vacuum Pressure – varied; Time of Transfer - 90 minutes; Gel percentage - 0.7%.
150
100
50
0
0.8 kb 4.1 kb 15 kb 29 kb
F ragment Size
3 in . H g
5 in . H g
7 in . H g
%
o
f
T
ra
n
sf
e
r
10 in . H g
120
100
80
60
40
20
0
0.8 kb 4.1 kb 15 kb 29 kb
F ragment Size
30 M inutes
90 M inutes
4 hours
%
o
f
T
ra
n
sf
e
r
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