Bio-Rad Model 785 Vacuum Blotter User Manual
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B. Equipment:
Comments/Setup
1. Model 785 Vacuum
Two or three units can be operated from the same
Blotter Unit
vacuum source. A three or four-way splitter can be
installed between the vacuum units and the liquid
trap. Each connector port from the splitter is
connected to the vacuum unit by vacuum tubing.
One port from the splitter is connected to the liquid
trap which in turn is connected to the Vacuum
Regulator. The Vacuum Regulator is connected to the
vacuum source.
2. Vacuum Regulator
Only one regulator is needed to run two to three
Vacuum Blotter units. Will not work efficiently with
too strong a vacuum pressure (³25 inches of Hg).
3. Vacuum sources
Bio-Rad’s Vacuum Pump, house vacuum, or vacuum
pumps can be used. A strong pump may require a
three-way valve to reduce the vacuum pressure. This
valve should be connected between the pump and the
Vacuum Regulator.
Caution: Do not apply ³ 25 inches of Hg to the
Vacuum Regulator.
4. Tygon tubing
1/4” inner diameter and 1/2” outer diameter. Offered
by Fisher Scientifics.
5. Liquid trap
1,500 ml-2,000 ml side-arm flask or commercially
available liquid trap.
6. Three or four-way splitter
Offered by the following venders: Fisher Scientific,
VWR, Baxter, and others.
7. Three-way valve
Offered by Fisher Scientific.
8. Plug
For side-arm flask. It should have a single hole that
fits a 5 ml pipet.
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