Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 107)

Quantity One User Guide
4-10
and removing less background. A smaller disk will more closely follow the profile
trace, removing more background.
A disk radius that is too large will result in poor removal of background. A disk radius
that is too small may subtract actual data.
Fig. 4-10. Examples of the background trace for small and large rolling disks. The small disk
follows the profile trace more closely, resulting in more background subtraction.
Select Lane Background from the Lane menu or toolbar and click on a lane. The
lane will be highlighted, the lane profile will be displayed, and the Lane Background
Subtraction dialog will open.
Small Radius Large Radius