Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 413)

Quantity One User Guide
K-16
Separate reference dark exposures will be taken for images that have different levels
of binning or gain. Once you have created a reference dark for each level of binning
or gain, the appropriate reference dark will be used according to the settings of your
selected application.
Reset Reference
If you would like a reference dark with an exposure time that more closely matches
that of your typical scans, click on the Reset Reference button.
A pop-up box will prompt you to enter a new dark exposure time in seconds.
Fig.K-9. Reset Reference Dark pop-up box.
Click on OK to implement your change. The new reference dark will be created when
you acquire your next image.
Because of the high sensitivity of the CCD, fluctuations in background radiation and/
or temperature in the room can affect the level of dark count. If you feel that radiation/
temperature conditions have changed in the room since your last reference dark was
created, use the Reset Reference button to delete your old reference and create a new
one under current conditions.
None
If you do not want to perform dark subtraction, select None. No dark exposure will be
acquired or subtracted.