Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 118)

5-1
5. Bands
After you have defined the lanes on the gel image, you can automatically identify and
quantitate bands using a set of adjustable parameters.
Note: You can quantitate bands, arrays, or other objects outside of lanes using volumes.
See Chapter 7 for details.
The tools for band detection are located on the Band menu and on the Band Tools
toolbar.
Fig. 5-1. Band menu and Band Tools toolbar.
Note: Before detecting bands, you should subtract background from the lanes using the
Lane Background command (see section 4.2, Lane-Based Background
Subtraction).