Bio-Rad Bio-Plex Pro™ Wash Stations - Manual (Page 49)

Defining New Programs
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 49
Washing Positions
Wash, dispense, and aspirate steps can be defined in a wash program with the
following Z-positions:
Overflow For ELISA assays and cellular assays. Overflow washing consists of a
simultaneous aspiration and dispense step. It creates a circular flow of wash
buffer in the well and ensures that the topmost parts of the well are also
washed.
Bottom For ELISA assays.
Custom Any necessary washing position can be selected; the custom positions are not
saved as *.pdf files in comparison to the overflow and bottom position.
Move Overflow If Move Overflow (MOVE OVER) is selected, the manifold moves step-wise
from the bottom position to the Overflow position during dispensing. It is
recommended for cell wash applications or any applications which must be
treated with care.
Move Custom If Move Custom (MOVE CUST.) is selected, the manifold moves step-wise
from the bottom position to the user-defined Custom Z- position during
dispensing. It is recommended for cell wash applications or any applications
which must be treated with care.
Z-Position Bottom
Z-Position Custom
Z-Position Overflow
Wash Position Diagrams
MOVE OVER.
Overflow Position
Movement of
manifold
MOVE CUST.
Custom Position
Movement of
manifold
Bottom Position Bottom Position
Movement Diagrams for Z-positions with MOVE