Bio-Rad Human MMP and TIMP Assays - Manual (Page 10)

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1. Plan Plate Layout
Prior to running the assay, determine the total number of wells in the
experiment using the Plate Layout Template on page 33 or the Plate
Formatting tab in Bio-Plex Manager
™ software. A suggested plate layout
is shown in Figure 2, with all conditions in duplicate.
1. Assign standards to columns 1 and 2, with the highest concentration
in row A and the lowest concentration in row H.
2. Assign the blank to wells A3 and A4. The blank should consist of your
chosen diluent HD or a diluent similar to your final sample type or
matrix. Note that Bio-Plex Manager automatically subtracts the blank
(B) MFI value from all other assay wells.
3. Controls, either user-specified or the controls supplied, are assigned to
wells in columns 3 and 4.
4. The remainder of the plate is available for samples.
5. once the total number of wells is known, calculate the required volumes
of beads, detection antibody, and streptavidin-PE needed. Use Tables 6,
8, and 9, respectively, or the Calculation Worksheet on page 34.
Legend
S Standard
B Blank
X Samples
C Controls
Fig. 2. Suggested plate layout. For detailed instructions on plate
formatting in Bio-Plex Manager software, see section Read Plate.