Bio-Rad Human MMP and TIMP Assays - Manual (Page 9)

7
lmportant Considerations
Instruments and Software
The Bio-Plex Pro
™ assays described in this manual are compatible with
all currently available Luminex-based life science research instruments.
Assays can be read and analyzed with either Bio-Plex Manager
™ software
or Luminex xPoNENT software (section 9).
Assay Procedures
Pay close attention to vortexing, shaking, and incubation times and to
Bio-Plex
® reader PMT (RP1) setting, as these have been optimized
specifically for each assay panel.
Assay Quick Guide
Each assay kit includes a printed Bio-Plex Pro Assay Quick Guide (bulletin
#10041638), which can be used to prepare and run a full 1 x 96-well assay
plate. Users can also download a copy at www.bio-rad.com/bio-plex.
Bead Regions
Bead regions for all analytes are listed in the Read Plate section.
Detailed Instructions
The following pages provide detailed instructions for each step of the
assay procedure, including preparation, running the assay, and reading
the plate with Bio-Plex Manager
™ and Luminex xPoNENT software.