Table of Contents
Introduction 1
Principle 2
Kit Contents and Storage
4
Recommended Materials
5
Assay Workflow
6
lmportant Considerations
7
Detailed Instructions
1.
1. Plan Plate Layout 8
2.
2. Prepare Instrument 9
3.
3. Prepare Wash Method 10
4.
4. Prepare Wash Buffer 11
5.
5. Prepare Standards and Controls 11
6. Prepare Samples 14
7.
7. Prepare Coupled Beads 16
8.
8. Run Assay 17
9.
9. Read Plate 21
Troubleshooting Guide
28
Plate Layout Template
33
Calculation Worksheet
34
Safety Considerations
35
Legal Notices
Ordering Information
36
