Bio-Rad Human MMP and TIMP Assays - Manual (Page 13)

11
Setting Up a Vacuum Manifold
Calibrate the vacuum manifold by placing a standard 96-well flat bottom
plate on the unit and adjusting the pressure to –1 to –3" Hg. In general,
100 µl liquid should take 3–4 sec to clear the well. For more detailed
instructions, refer to bulletin #10005042.
4. Prepare Wash Buffer
1. Bring the 10x stock solution to room temperature.
2. If crystals exist, ensure that they are completely dissolved. Mix the
10x stock solution by inversion before preparing the 1x wash buffer.
3. To prepare 1x wash buffer, dilute 1 part 10x stock solution with
9 parts deionized water.
5. Prepare Standards and Controls
General Instructions
n
It is essential to prepare standards and controls exactly as described
in this section. Incorrect preparation may lead to low signal or variable
measurements from plate to plate
n
The product data sheet provided lists the most concentrated point on the
standard curve (S1). Enter the values and units into Bio-Plex Manager ™
software as instructed in section 9
Using the Controls (optional)
one vial of controls is included. Its use is intended for monitoring the
day-to-day quality of assay results.