Table of Contents
Introduction 1
Principle 2
Kit Contents and Storage
4
Recommended Materials
5
Assay Workflow
6
lmportant Considerations
7
Detailed Instructions
1. Plan Plate Layout 82. Prepare Instrument 93. Prepare Wash Method 104. Prepare Standards and Controls 115. Prepare Samples 136. Prepare Coupled Beads 167. Run Assay 178. Read Plate 21
Troubleshooting Guide
29
Plate Layout Template
34
Calculation Worksheet
35
Safety Considerations
37
Legal Notices
Ordering Information
38