Controls, And samples – Bio-Rad Bio-Plex Pro™ Human Cancer Biomarker Assays User Manual
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3. Format the plate in Bio-Plex Manager according to the plate layout
created in section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 4).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all
the wells that contain standards. Repeat this process for
Blanks
B
, Controls
C
, and Samples
X
. Note that
Bio-Plex Manager automatically subtracts the blank FI
value from all other assay wells.
Table 13. Bead regions for human cancer biomarker panels.
Panel 1
Analyte
Bead Region
Analyte
Bead Region
Analyte
Bead Region
sEGFR 15
sIL-6R
a
19
Prolactin
52
FGF-basic
44
Leptin
78
SCF
65
Follistatin
26
Osteopontin
77
TIE-2
64
G-CSF
57
PDGF-AB/BB 47
sVEGFR-1
76
sHER2/neu
12
PECAM-1
46
sVEGFR-2
45
HGF
62
Panel 2
Angiopoietin-2 47
IGFBP-1
62
TGF-
a 76
sCD40L 29
IL-6
19
TNF-
a 36
EGF 14 IL-8 54 uPA 78
Endoglin 26
IL-18
42
VEGF-A 45
sFASL 35
PAI-1
61
VEGF-C 64
HB-EGF 57
PLGF 52
VEGF-D 43